最近発表した論文
1.Jia, N., Semba, U., Nishiura, H., Kuniyasu, A., Nsiama, T.K., Nishino, N., and Yamamoto, T. Pivotal Advance: Interconversion between pure chemotactic ligands and chemoattractant/secretagogue ligands of neutrophil C5a receptor by a single amino acid substitution. J Leukoc Biol. (2010) 87, 965-975
2. Murata K., Nishimura S., Kuniyasu A., and Nakayama H. Three-dimensional structure of alpha1-beta complex in skeletal muscle dihydropyridine receptor by single particle electron microscopy. J. Electron Microsc. (2010) 59, 215-216
3. Nambu T., Araki N., Nakagawa A., Kuniyasu A., Kawaguchi T., Hamada A., and Saito H. Contribution of BCR-ABL-independent Activation of ERK1/2 to Acquired Imatinib Resistance in K562 Chronic Myeloid Leukemia Cells. Cancer Sci. (2010) 101, 137–142
4. 國安 明彦, オートファジーを誘導するペプチド, ケミカルエンジニヤリング (2009) 54, 819-824
5.Kawahara, K., Nishi, K., Suenobu, M., Ohtsuka, H., Maeda, A., Nagatomo, K., Kuniyasu, A., Staufenbiel, M., Nakagomi, M., Shudo, K., and Nakayama, H. Oral administration of synthetic retinoid Am80 (tamibarotene) decreases brain β-amyloid peptides in APP23 mice. Biol. Pharm. Bull. (2009) 32, 1307-1309
6. Kawahara, K., Yoshida, A., Koga, K., Yokoo, S., Kuniyasu, A., Gotoh, T., Sawada, M., and Nakayama, H. Marked induction of inducible nitric oxide synthase and tumor necrosis factor-α in rat CD40+ microglia by comparison to CD40- microglia. J. Neuroimmunol. (2009) 208, 70-79
7. Yokote, S., Setoguchi, R., Shimizu, E., Kawahara, K., Kuniyasu, A., Shirasaki, T., Takahama, K., Konno, K., Kawai, N., Yamaoka, K., Kinoshita, E., and Nakayama, H. A synthetic approach to develop peptide inhibitors delective for brain-type sodium channels on the basis of pompilidotoxin structure. Heterocycles (2009) 79, 925-933
8.Makise, M., Takehara, M., Kuniyasu, A., Matsui, N., Nakayama, H., and Mizushima, T. Linkage between phosphorylation of the origin recognition complex and its ATP binding activity in Saccharomyces cerevisiae. J. Biol. Chem. (2009) 284, 3396-3407.
9.Shimizu, E., Kawahara, K., Kajizono, M., Sawada, M., and Nakayama, H. Interleukin-4-induced selective clearance of oligomeric β-amyloid1-42 by rat type 2 microglia. J. Immunol. (2008) 181, 6503-6513
10.Nishimura S, Takahashi S, Kamikatahira H, Kuroki Y, Jaalouk DE, O'Brien S, Koivunen E, Arap W, Pasqualini R, Nakayama H, and Kuniyasu A. Combinatorial targeting of the macropinocytotic pathway in leukemia and lymphoma cells. J. Biol. Chem. (2008) 284, 3396-3407.
11. Kaneko, K., Fukuda, H., Chuang, V. T., Yamasaki, K., Kawahara, K., Nakayama, H., Suenaga, A., Maruyama, M., and Otagiri, M. Subdomain IIIA of dog albumin contains a binding site similar to site II of human albumin. Drug Metab. Dispos., (2008) 36, 81-86
12.Hirayama C, Watanabe H, Nakashima R, Nanbu T, Hamada A, Kuniyasu A, Nakayama H, Kawaguchi T, Saito H.: Constitutive overexpression of P-glycoprotein, rather than breast cancer resistance protein or organic cation transporter 1, contributes to acquisition of imatinib-resistance in K562 cells. Pharm. Res. (2008) 827-835
13. Kaneko K, Akuta T, Sawa T, Kim HW, Fujii S, Okamoto T, Nakayama H, Ohigashi H, Murakami A, Akaike T.: Mutagenicity of 8-nitroguanosine, a product of nitrative nucleoside modification by reactive nitrogen oxides, in mammalian cells. Cancer Lett. (2008) 262 (2), 239-247.
14. Bover LC, Cardo-Vila M, Kuniyasu A, Sun J, Rangel R, Takeya M, Aggarwal BB, Arap W, Pasqualini R.: A previously unrecognized protein-protein interaction between TWEAK and CD163: potential biological implications. J. Immunol. (2007) 178 (12), 8183-8194
過去の代表的論文
１． イオンチャネルの構造と機能の解析
(1) A. Kuniyasu, K. Itagaki, T. Shibano, M. Iino, G. Kraft, A. Schwartz
and
H. Nakayama:
.: Photochemical Identification of Transmembrane Segment IVS6 as the Binding
Region of Semotiadil, a New Modulator for the L-type Voltage Dependent
Ca2+ Channel: J. Biol. Chem., 273, 4635-4641 (1998).
(2) M. Ohkura, K. Furukawa, H. Fujimori,
A. Kuruma, S. Kawano, M. Hiraoka,
A. Kuniyasu, H. Nakayama and Y. Ohizumi:
.:Dual Regulation of the Skeletal Muscle Ryanodine Receptor by Triadin
and
Calsequestrin. Biochemistry, 37, 12987-12993 (1998).
(3) N. Ii, A. Kuniyasu, K. Kawahara, T. Shibano, A. Schwartz and H. Nakayama.:
Photochemical Localization of the Semotiadil Binding Region within Cardiac
Ca2+ Channel a1 Subunit. Comparison with the Skeletal Muscle Counterpart.
FEBS Letters, 441, 83-87 (1998).
(4) A. Kuniyasu, S. Kawano, Y. Hirayama, Y.
-H. Ji, K. Xu, M. Ohkura,
K. Furukawa, Y. Ohizumi, M. Hitaoka and H. Nakayama.:
A New Scorpion Toxin Stimulates the Ca2+-Release Channel Activity of
the Skeletal Muscle Ryanodine Receptors by an Indirect Mechanism.
Biochem. J., 339, 343-350 (1999).
(5) K. Murata, N. Odahara, A. Kuniyasu, Y. Sato, H. Nakayama and K. Nagayama.:
Asymmetric Arrangement of Auxiliary Subunits of Skeletal Muscle Voltage-Gated
L-Type Ca2+ Channel. Biochem. Biophys. Res. Commun., 282, 284-291 (2001).
(6) E. Kinoshita, H.
Maejima, K. Yamaoka, K. Konnno, N. Kawai, E. Shimizu, S. Yokote,
H. Nakayama and I. Seyama.:
A Novel Wasp Toxin Discriminates between Neuronal and Cardiac Sodium Channel.
Mol. Pharmacol., 59, 1457-1463 (2001).
(7)Yoshikawa H, Shimizu E, Kawahara K, Kuniyasu A, Shibano T and Nakayama
H.:
Photochemical identification of the binding region for (S)-semotiadil
on sodium channels:
comparison with that for (R)-semotiadil on skeletal muscle calcium channel.
Heterocycles, 59, 259-263 (2003)
(8)Kuniyasu A, Kaneko K, Kawahara K and Nakayama H.:
Molecular assembly and subcellular distribution of ATP-sensitive potassium
channnel proteins in rat hearts.
FEBS Lett.., 552, 613-622 (2003)
２．疾患とイオンチャネル、レセプター
(1) M. Akao, H. Otani, M.
Horie, M. Takano, A. Kuniyasu, H. Nakayama,
I. Kouchi, S. Sasayama and T. Murakami.:
Myocardial Ischemia Induces Differential Regulation of KATP Channel Gene
Expression in Rat Hearts. J. Clin. Invest., 100, 3053 - 3059 (1997).
(2) K. Anzai, K. Ogawa, A. Kuniyasu, T. Ozawa, H. Yamamoto and H. Nakayama.:
Effects of Hydroxyl Radicals and Sulfhydryl Reagents on the Open Probability
of the Purified Cardiac Ryanodine Receptor Channel Incorporated into Planar
Lipid Bilayers. Biochem. Biophys. Res. Commun., 249, 938-942 (1998).
(3) H. K. Motoike, I. Bodi, H. Nakayama, A. Schwartz and G. Varadi.:
A Region in IVS5 of the Human Cardiac L-type Calcium Channel Is Required
for the Use-dependent Block by Phenylalkylamines and Benzothiazepines.
J. Biol. Chem., 274, 9409-9420 (1999).
３． 糖尿病、動脈硬化の分子機構と薬物開発
(1) N. Ohgami, A. Kuniyasu, K. Furukawa, A. Miyazaki, H.
Hakamata, S. Horiuchi
and H. Nakayama.:
Glibenclamide Acts as an Inhibitor of Acyl-CoA:Cholesterol Acyltransferase
Enzyme. Biochem. Biophys. Res. Commun., 277, 417-422 (2000).
(2) N. Ohagami, R. Nagai, M. Ikemoto, H. Arai, A.
Kuniyasu, S. Horiuchi
and H. Nakayama.:
CD36, a Member of Class B Scavenger Receptor Family, as a Receptor for
Advanced Glycation End Products (AGE). J. Biol. Chem., 276, 3195-3202
(2001).
(3) N. Ohagami, R. Nagai, A.
Miyazaki, M. Ikemoto, H. Arai, S. Horiuchi
and H. Nakayama.:
Scavenger Receptor Class B Type I-Mediated Reverse Cholesterol Transport
is
Inhibited by Advanced Glycation End Products. J. Biol. Chem., 276, 13348-13355
(2001).
(4) Ohgami N, Nagai R, Ikemoto M, Arai H, Kuniyasu A, Horiuchi S and Nakayama
H.:
CD36, a member of class B scavenger receptor family, is a receptor for
advanced glycation end products.
Ann. N Y Acad. Sci., 947, 350-355 (2001)
(5) Ohgami N, Nagai R, Ikemoto M, Arai H, Miyazaki A, Hakamata H, Horiuchi S and Nakayama H.:
CD36, serves as a receptor for advanced glycation endproducts (AGE).
J. Diabetes Complications, 16, 56-59 (2002)
(6) Miyazaki A, Nakayama H and Horiuchi S:
Scavenger receptors that recognize advanced glycation end products.
Trends Cardiovasc. Med., 12, 258-262 (2002)
(7) Kuniyasu A, Hayashi S and Nakayama H.:
Adipocytes recognize and degrade oxidized low density lipoprotein through
CD36.
Biochem. Biophys. Res. Commun. , 295, 319-323 (2002)
(8) Ohgami N, Miyazaki A, Sakai M, Kuniyasu A, Nakayama H and Horiuchi
S.:
Advanced Glycation End Products (AGE) Inhibit Scavenger Receptor Class
B type I-Mediated
Reverse Cholesterol Transport: a New Crossroad of AGE to Cholesterol Metabolism.
J Atheroscler Thromb., 10, 1-6 (2003)
(9) Kuniyasu A, Ohgami N, Hayashi S, Miyazaki A, Horiuchi S and Nakayama H.:
CD36-mediated endocytic uptake of advanced glycation end products (AGE)
in mouse 3T3-L1 and
human subcutaneous adipocytes.
FEBS Lett., 537, 85-90 (2003)
(10) Furukawa K, Hori M, Ouchi N, Kihara S,
Funahashi T, Masuzawa Y, Miyazaki A,
Nakayama H and Horiuchi S.:
adiponection down-regulates acyl-coenzyme A:
cholesterol acyltransferase-1 in cultured human monocye-derived macrophages.
Biochem.Biophys.Res.Commun. , 317, 831-836(2004)
４．ミクログリアにおけるＮＯの役割
(1) K. Kawahara, T. Gotoh, S. Oyadomari, M.
Kajizono, A. Kuniyasu, K. Ohsawa,
Y. Imai, S. Kohsaka, H. Nakayama and M. Mori.:
Co-Induction of Argininosuccinate Synthease, Cationic Amino Acid Transporter-2
and Nitric Oxide Synthase in Activated Murine Microglial Cells.
Mol. Brain Res., 90, 165-173 (2001).
(2) K. Kawahara, T. Gotoh, S. Oyadomari, A. Kuniyasu, S. Kohsaka, M. Mori
and H. Nakayama.:
Nitric Oxide Inhibits the Proliferation of Murine Microglial MG5 Cells
by a Mechanism
Involving p21 but Independent of p53 and Cyclic Guanosine Monophosphate.
Neurosci.Lett., 310, 89-92 (2001).
(3) Kawahara K, Oyadomari S, Gotoh T, Kohsaka S, Nakayama H and Mori M.:
Induction of CHOP and apoptosis by nitric oxide in p53-deficient microglial
cells.
FEBS Lett., 506, 135-139 (2001)
５．分子プローブと生体高分子の認識機構
(1) S. Takeda, S. Miyauchi, H. Nakayama and N. Kamo.:
Adenosine 5'-Triphosphate Binding to Bovine Serum Albumin.
Biophys. Chem., 70, 175-183 (1997).
(2) L.- C. Xu, M. Nakayama, K. Harada, H.
Nakayama, S. Tomiguchi, A. Kojima,
M. Takahashi and Y. Arano.:
Synthesis and Evaluation of Hydroxamide-Based Tetradentate Ligands as
a
New Class of Thiol-Free Chilating Molecules for 99mTc-radiopharmaceu-
ticals. Nucl. Med. Biol., 25, 295-303 (1998).
(3) L.-C. Xu, M. Nakayama, K. Harada, A. Kuniyasu, H. Nakayama,
S. Tominaga, A. Kojima, M. Takahashi, M. Ono, Y. Arano, H. Saji, Z. Yao,
H. Sakahara, and J. Konishi.:
Bis(Hydroxamamide)-Based Bifunctional Chelating Agent for 99mTc
Labeling of Polypeptides. Bioconjugate Chem., 10, 9-17 (1999).
(4) V. T. Chuang, A. Kuniyasu, H. Nakayama, Y.
Matsushita, S. Hirono and
M. Otagiri.:
Helix 6 of Subdomain IIIA of Human Serum Albumin Is the Region Primarily
Photolabeled by Ketoprofen, An Arylpropionic acid NSAID Containing a
Benzophenone Moiety. Biochim. Biophys. Acta, 1434, 18-30 (1999).
(5) K. Furuta, T. Hosoya, K. Tomikiyo, S. Okuda,
A. Kuniyasu, H. Nakayama,
T. Ishikawa and M. Suzuki.:
[35S]S-[5-(Benzoylphenyl)pentyl]glutathione: A Versatile Radiligand
TargetingGS-X Pump with the Ability of Photoaffinity Labeling.
Bioorg. Med. Chem. Lett., 9, 2661-2666 (1999).
(6) Z.-H. Lei, A. Kuniyasu, B. -S. Tai, H. Nakayama, and T. Nohara.:
Na+,
K+-ATPase inhibiting Activity of Cardiac Glycosides from
Erysimum
Cheirantoides. Planta Medica , 67, 369-370 (2001)
(7) Harada K, Kuniyasu A, Nakayama H, Nakayama M, Matsunaga T, Uji Y, Sugiuchi
H and Okabe H.:
Separation of human serum transferrins with different iron-binding states
by high-performance
liquid chromatography using a pyridinium polymer column.
J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 767, 45-51 (2002)
(8) Kawahara K, Kuniyasu A, Masuda K, Ishiguro M and Nakayama H.:
Efficient identification of photolabelled amino acid residues by combining
immunoaffinity purification
with MS: revealing the semotiadil-binding site and its relevance to binding
sites for myristates in domain III
of human serum albumin.
Biochem. J., 363, 223-232 (2002)
(9) Lei ZH, Nakayama H, Kuniyasu A, Tai BS and Nohara T.:
Cardiac Glycosides from Erysimum cheiranthoides.
Chem. Pharm. Bull (Tokyo), 50,861-862 (2002)
(10) Usami N, Ishikura S,
Abe H, Nagano M, Uebuchi M, Kuniyasu A, Otagiri M, Nakayama H, Imamura Y
and Hara A.:
Cloning, expression and tissue distribution of a tetrameric form of pig
carbonyl reductase.
Chem. Biol. Interact., 143-144, 353-361 (2003)
(11) Goto M, Yasuzawa
H, Higashi T, Yamaguchi Y, Kawanami A, Mifune S, Mori H,
Nakayama H, Harada K and Arakawa Y.:
Dependence of hydrolysis of b-lactams with a Zn(II) from serratia marcescens(IMP-1)
on pH and
concentration of Zn(II) ion:
Dissociation of Zn(II) from IMP-1 in acidic medium.
Biol.Pharm.Bull J., 26, 589-594 (2003)
(12) Nakayama M, Haratake
M, Ono M, Koiso T, Harada K, Nakayama H, Yahara S,
Ohmomo Y and Arano Y.:
A new 68 Ge/68 Ga generator system using an organic polymer containing
N-Methylglucamine groups as adsorbent for 68 Ge
Appl Radiat Isot. , 58, 9-14 (2003)
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